Vibrio cholerae can cause syndromes from asymptomatic to cholera gravis. Symptoms include abrupt onset of watery diarrhoea, occasional vomiting and abdominal cramps. Dehydration ensues with symptoms and signs such as thirst, dry mucous membranes, decreased skin turgor, sunken eyes, hypotension, weak pulse, tachycardia, tachypnea, hoarse voice, oliguria, cramps, renal failure, seizures, somnolence, coma and death.
AmpliSens® Vibrio cholerae-FRT PCR kit enables to detect V. cholerae DNA (if Hly sequence is present) and identification of pathogen V. cholerae strains (if main virulence factors - CtxA, tcpA are present), belonging to serogroups O1 (if amplification target wbeT is present), or O139 (if amplification target wbf is present). Analytical sensitivity is 1 x 103 copies/ml.
AmpliSens® Vibrio cholerae-FRT PCR kit contains two mastermixes: PCR-mix-1-FRT screen for amplification of CtxA target, tcpA and Internal Control, and the PCR-mix-1-FRT type for amplification of Hly target (cholera germs of all group), wbeT (serogroup O1) and wbf (serogroup O139). It is necessary to carry out both “Screen” and “Type” reactions for valid results interpretation.