Rubella virus belongs to the family Togaviridae. It causes a mild infection characterized by a rash starting on the face and gradually spreading to the feet, fever, lymphadenopathy and other flu-like symptoms such as coughing, sore throat and sneezing. Older children and adults may experience joint involvement and purpuric rash. Women in their first trimester who contract rubella have an increased risk of passing the infection to the developing foetus. When contracted during the first trimester the effects on the child are most marked. Ocular, cardiovascular and central nervous system defects are common, along with deafness and intrauterine growth retardation. Second trimester infections are associated with deafness, retinopathy, microcephaly and mental retardation, while third trimester infections are associated with intrauterine growth retardation.
AmpliSens® Rubella virus-FRT PCR kit is One-Step RT-PCR kit based on RNA extraction (plasma, saliva, throat swabs, amniotic fluid), followed with reverse transcription (RT kit is included) and cDNA amplification. Internal Control allows to control RNA extraction efficiency, as well as RT and PCR processes.