HIV Infection

Human immunodeficiency virus (HIV) is a lentivirus that causes acquired immunodeficiency syndrome (AIDS), a condition in humans, in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. There are two types of HIV - HIV-1 and HIV-2.

Usually, unless otherwise noted, the term HIV primarily refers to HIV-1. Both types of HIV damage a person’s body by destroying specific blood helper T cells (CD4+) HIV infects also other vital cells in the human immune system such as macrophages and dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: first - the direct viral killing of infected cells; second - increased rates of apoptosis in infected cells and third - the killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost and the body becomes progressively more susceptible to opportunistic infections. Most untreated people infected with HIV-1 develop AIDS. These individuals mostly die from opportunistic infections or malignancies associated with the progressive failure of the immune system.

AmpliSens® DNA-HIV-FRT PCR kit is a qualitative DNA test based on the amplification of HIV DNA target region and Internal Control. Such Internal Control allows us to determine the quality of DNA extraction and amplification processes. Analytical sensitivity is 500 GE/ml DNA (250 μl sample).

AmpliSens® HIV-Monitor-FRT PCR kit is One-Step RT-PCR test for qualitative detection and quantitation of HIV type 1 RNA in the clinical material (plasma). The RNA based kits contain Internal Control that allows to determine the quality of RNA extraction, reverse transcription and amplification processes. Analytical sensitivity is 500 copies/ml HIV-1 (100 μl sample) or 50 copies/ml HIV-1 (1 ml sample).

Catalog   number	Description	Detection	Format	# of reactions	Certifi- cation	Assay detail	Shelf life	Fluorescent channels	Availa- bility
R-V0-MC (RG, iQ, Mx, Dt)-CE	AmpliSens® HIV-Monitor-FRT PCR kit			80		quantitative	12 months
TR-V0-G (RG, iQ)-CE	AmpliSens® DNA-HIV-FRT*			120		qualitative	12 months
NEW H-4011-1-14-CE	AmpliSens® HIV-Monitor-L PCR kit variant FRT-L			480		quantitative	12 months
NEW H-4011-1-14-100-CE	AmpliSens® HIV-Monitor-L PCR kit variant FRT-L			96		quantitative	12 months

*in regards to transport conditions, this product is classified as dangerous goods


  Real-Time     Reverse transcription    Fluorescent End-Point     Reagents in stock tubes    Ready-to-use PCR tubes   
 on request     available