Lyme disease is caused by the bacterium Borrelia burgdorferi and is transmitted to humans through the bite of infected black-legged ticks. Typical symptoms include fever, headache, fatigue and a skin rash called erythema migrans. If left untreated, infection can spread to joints, heart and nervous system. Lyme disease diagnostics is based on symptoms, physical findings (e.g. rash) and the possibility of exposure to infected ticks.
AmpliSens® Borrelia burgdorferi sensu lato-FRT PCR kit is amplification test for the qualitative detection of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. garinii) 16S rRNA in the biological material. Kit is based on RNA extraction, reverse transcription and amplification of target RNA region. Such RNA detection is much effective and much sensitive than if detection is based only on DNA analysis.